Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea

Huiluo Cao; Yiguo Hong; Meng Li; Ji Dong Gu

doi:10.1007/s10482-011-9610-1

Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea

Huiluo Cao, Yiguo Hong, Meng Li, Ji Dong Gu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

In the present study the diversity and abundance of nitrifying microbes including ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) were investigated, along with the physicochemical parameters potentially affecting them, in a transect of surface sediments from the coastal margin adjacent to the Pearl River estuary to the slope in the deep South China Sea. Nitrifying microbial diversity was determined by detecting the amoA (ammonia monooxygenase subunit A) gene. An obvious community structure shift for both AOA and beta-AOB from the coastal marginal areas to the slope in the deep-sea was detected, while the OTU numbers of AOA amoA were more stable than those of the beta-AOB. The OTUs of beta-AOB increased with the distance from the coastal margin areas to the slope in the deep-sea. Beta-AOB showed lower diversity with dominant strains in a polluted area but higher diversity without dominant strains in a clean area. Moreover, the diversity of beta-AOB was correlated with pH values, while no noticeable relationships were established between AOA and physicochemical parameters. Beta-AOB was more sensitive to transect environmental variability and might be a potential indicator for environmental changes. Additionally, the surface sediments surveyed in the South China Sea harboured diverse and distinct AOA and beta-AOB phylotypes different from other environments, suggesting the endemicity of some nitrifying prokaryotes in the South China Sea.

Original language	English
Pages (from-to)	545-556
Number of pages	12
Journal	Antonie van Leeuwenhoek
Volume	100
Issue number	4
DOIs	https://doi.org/10.1007/s10482-011-9610-1
State	Published - Nov 2011
Externally published	Yes

Keywords

Ammonia monooxygenase subunit A (amoA)
Ammonia-oxidizing archaea
Ammonia-oxidizing bacteria
South China Sea

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10.1007/s10482-011-9610-1

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@article{8fdf3d0b07b64f4491c8a23a0026ebd1,

title = "Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea",

abstract = "In the present study the diversity and abundance of nitrifying microbes including ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) were investigated, along with the physicochemical parameters potentially affecting them, in a transect of surface sediments from the coastal margin adjacent to the Pearl River estuary to the slope in the deep South China Sea. Nitrifying microbial diversity was determined by detecting the amoA (ammonia monooxygenase subunit A) gene. An obvious community structure shift for both AOA and beta-AOB from the coastal marginal areas to the slope in the deep-sea was detected, while the OTU numbers of AOA amoA were more stable than those of the beta-AOB. The OTUs of beta-AOB increased with the distance from the coastal margin areas to the slope in the deep-sea. Beta-AOB showed lower diversity with dominant strains in a polluted area but higher diversity without dominant strains in a clean area. Moreover, the diversity of beta-AOB was correlated with pH values, while no noticeable relationships were established between AOA and physicochemical parameters. Beta-AOB was more sensitive to transect environmental variability and might be a potential indicator for environmental changes. Additionally, the surface sediments surveyed in the South China Sea harboured diverse and distinct AOA and beta-AOB phylotypes different from other environments, suggesting the endemicity of some nitrifying prokaryotes in the South China Sea.",

keywords = "Ammonia monooxygenase subunit A (amoA), Ammonia-oxidizing archaea, Ammonia-oxidizing bacteria, South China Sea",

author = "Huiluo Cao and Yiguo Hong and Meng Li and Gu, {Ji Dong}",

note = "Funding Information: Acknowledgments This research was supported by a PhD studentship (H-L C) from The University of Hong Kong and in part by grants from Agriculture, Fisheries and Conservation Department of the Hong Kong SAR Government (J-DG), and National Natural Science Foundation of China (41076095), Knowledge Innovation Key Project of the Chinese Academy of Sciences (KZCX2-YW-QN207) (Y-GH). We would like to thank Ms. Jessie Lai and Dr Zhenye Zhao for their kindly laboratory assistances throughout this investigation at The University of Hong Kong.",

year = "2011",

month = nov,

doi = "10.1007/s10482-011-9610-1",

language = "英语",

volume = "100",

pages = "545--556",

journal = "Antonie van Leeuwenhoek",

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T1 - Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea

AU - Cao, Huiluo

AU - Hong, Yiguo

AU - Li, Meng

AU - Gu, Ji Dong

N1 - Funding Information: Acknowledgments This research was supported by a PhD studentship (H-L C) from The University of Hong Kong and in part by grants from Agriculture, Fisheries and Conservation Department of the Hong Kong SAR Government (J-DG), and National Natural Science Foundation of China (41076095), Knowledge Innovation Key Project of the Chinese Academy of Sciences (KZCX2-YW-QN207) (Y-GH). We would like to thank Ms. Jessie Lai and Dr Zhenye Zhao for their kindly laboratory assistances throughout this investigation at The University of Hong Kong.

PY - 2011/11

Y1 - 2011/11

N2 - In the present study the diversity and abundance of nitrifying microbes including ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) were investigated, along with the physicochemical parameters potentially affecting them, in a transect of surface sediments from the coastal margin adjacent to the Pearl River estuary to the slope in the deep South China Sea. Nitrifying microbial diversity was determined by detecting the amoA (ammonia monooxygenase subunit A) gene. An obvious community structure shift for both AOA and beta-AOB from the coastal marginal areas to the slope in the deep-sea was detected, while the OTU numbers of AOA amoA were more stable than those of the beta-AOB. The OTUs of beta-AOB increased with the distance from the coastal margin areas to the slope in the deep-sea. Beta-AOB showed lower diversity with dominant strains in a polluted area but higher diversity without dominant strains in a clean area. Moreover, the diversity of beta-AOB was correlated with pH values, while no noticeable relationships were established between AOA and physicochemical parameters. Beta-AOB was more sensitive to transect environmental variability and might be a potential indicator for environmental changes. Additionally, the surface sediments surveyed in the South China Sea harboured diverse and distinct AOA and beta-AOB phylotypes different from other environments, suggesting the endemicity of some nitrifying prokaryotes in the South China Sea.

AB - In the present study the diversity and abundance of nitrifying microbes including ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) were investigated, along with the physicochemical parameters potentially affecting them, in a transect of surface sediments from the coastal margin adjacent to the Pearl River estuary to the slope in the deep South China Sea. Nitrifying microbial diversity was determined by detecting the amoA (ammonia monooxygenase subunit A) gene. An obvious community structure shift for both AOA and beta-AOB from the coastal marginal areas to the slope in the deep-sea was detected, while the OTU numbers of AOA amoA were more stable than those of the beta-AOB. The OTUs of beta-AOB increased with the distance from the coastal margin areas to the slope in the deep-sea. Beta-AOB showed lower diversity with dominant strains in a polluted area but higher diversity without dominant strains in a clean area. Moreover, the diversity of beta-AOB was correlated with pH values, while no noticeable relationships were established between AOA and physicochemical parameters. Beta-AOB was more sensitive to transect environmental variability and might be a potential indicator for environmental changes. Additionally, the surface sediments surveyed in the South China Sea harboured diverse and distinct AOA and beta-AOB phylotypes different from other environments, suggesting the endemicity of some nitrifying prokaryotes in the South China Sea.

KW - Ammonia monooxygenase subunit A (amoA)

KW - Ammonia-oxidizing archaea

KW - Ammonia-oxidizing bacteria

KW - South China Sea

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DO - 10.1007/s10482-011-9610-1

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C2 - 21717206

AN - SCOPUS:80054098914

SN - 0003-6072

VL - 100

SP - 545

EP - 556

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

IS - 4

ER -

Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea

Abstract

Keywords

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