Diversity and abundance of ammonia-oxidizing archaea and bacteria in polluted mangrove sediment

TY - JOUR

T1 - Diversity and abundance of ammonia-oxidizing archaea and bacteria in polluted mangrove sediment

AU - Cao, Huiluo

AU - Li, Meng

AU - Hong, Yiguo

AU - Gu, Ji Dong

N1 - Funding Information: This research was supported in part by grants from Agriculture, Fisheries and Conservation Department of the Hong Kong Government (J-D G), a PhD studentship (H-LC); and National Natural Science Foundation of China ( 41076095 ), and Knowledge Innovation Key Project of the Chinese Academy of Sciences ( KZCX2-YW-QN207 ) (Y-G H). We thank Dr. Zhenye Zhao for the help in the fieldwork and Jessie Lai for the laboratory assistance at The University of Hong Kong.

PY - 2011/11

Y1 - 2011/11

N2 - Ammonia oxidation by microorganisms is a critical process in the nitrogen cycle. Recent research results show that ammonia-oxidizing archaea (AOA) are both abundant and diverse in a range of ecosystems. In this study, we examined the abundance and diversity of AOA and ammonia-oxidizing beta-proteobacteria (AOB) in estuarine sediments in Hong Kong for two seasons using the ammonia monooxygenase A subunit gene (amoA) as molecular biomarker. Relationships between diversity and abundance of AOA and AOB and physicochemical parameters were also explored. AOB were more diverse but less abundant than AOA. A few phylogenetically distinct amoA gene clusters were evident for both AOA and AOB from the mangrove sediment. Pearson moment correlation analysis and canonical correspondence analysis (CCA) were used to explore physicochemical parameters potentially important to AOA and AOB. Metal concentrations were proposed to contribute potentially to the distributions of AOA while total phosphorus (TP) was correlated to the distributions of AOB. Quantitative PCR estimates indicated that AOA were more abundant than AOB in all samples, but the ratio of AOA/AOB (from 1.8 to 6.3) was smaller than most other studies by one to two orders. The abundance of AOA or AOB was correlated with pH and temperature while the AOA/AOB ratio was with the concentrations of ammonium. Several physicochemical factors, rather than any single one, affect the distribution patterns suggesting that a combination of factors is involved in shaping the dynamics of AOA and AOB in the mangrove ecosystem.

AB - Ammonia oxidation by microorganisms is a critical process in the nitrogen cycle. Recent research results show that ammonia-oxidizing archaea (AOA) are both abundant and diverse in a range of ecosystems. In this study, we examined the abundance and diversity of AOA and ammonia-oxidizing beta-proteobacteria (AOB) in estuarine sediments in Hong Kong for two seasons using the ammonia monooxygenase A subunit gene (amoA) as molecular biomarker. Relationships between diversity and abundance of AOA and AOB and physicochemical parameters were also explored. AOB were more diverse but less abundant than AOA. A few phylogenetically distinct amoA gene clusters were evident for both AOA and AOB from the mangrove sediment. Pearson moment correlation analysis and canonical correspondence analysis (CCA) were used to explore physicochemical parameters potentially important to AOA and AOB. Metal concentrations were proposed to contribute potentially to the distributions of AOA while total phosphorus (TP) was correlated to the distributions of AOB. Quantitative PCR estimates indicated that AOA were more abundant than AOB in all samples, but the ratio of AOA/AOB (from 1.8 to 6.3) was smaller than most other studies by one to two orders. The abundance of AOA or AOB was correlated with pH and temperature while the AOA/AOB ratio was with the concentrations of ammonium. Several physicochemical factors, rather than any single one, affect the distribution patterns suggesting that a combination of factors is involved in shaping the dynamics of AOA and AOB in the mangrove ecosystem.

KW - Ammonia-oxidizing archaea

KW - Ammonia-oxidizing bacteria

KW - AmoA

KW - Mangrove sediment

KW - Q-PCR

UR - http://www.scopus.com/inward/record.url?scp=79955513133&partnerID=8YFLogxK

U2 - 10.1016/j.syapm.2010.11.023

DO - 10.1016/j.syapm.2010.11.023

M3 - 文章

C2 - 21665398

AN - SCOPUS:79955513133

SN - 0723-2020

VL - 34

SP - 513

EP - 523

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 7

ER -