TY - JOUR
T1 - Direct imaging and computational cryo-electron microscopy of ribbons and nanotubes
AU - Danino, Dganit
AU - Egelman, Edward H.
N1 - Publisher Copyright:
© 2018
PY - 2018/3
Y1 - 2018/3
N2 - Ribbons, filaments (fibers) and nanotubes are common structures in biological systems. They form by lipids, peptides and proteins, and are associated with many fundamental processes such as shaping membrane curvature, cell signaling, and protein function and dysfunction. Identical morphologies formed by natural or synthetic building blocks find numerous applications, e.g., in regenerative medicine, medical therapeutics, templating, catalysis and energy storage devices. Although the scientific context and chemistry of the building blocks may vary considerably, the forces that drive their formation, the local interactions, the structure and the morphology are closely related. Cryo-EM methodologies (Cryo-TEM in the colloid science community) have proven powerful in resolving structure-function-property relations of these macromolecular assemblies. The present review contains two main sections highlighting the unique cryo-EM contribution to understanding the global and the local features of nano-fibers, ribbons and tubes, as well as their detailed high-resolution structure.
AB - Ribbons, filaments (fibers) and nanotubes are common structures in biological systems. They form by lipids, peptides and proteins, and are associated with many fundamental processes such as shaping membrane curvature, cell signaling, and protein function and dysfunction. Identical morphologies formed by natural or synthetic building blocks find numerous applications, e.g., in regenerative medicine, medical therapeutics, templating, catalysis and energy storage devices. Although the scientific context and chemistry of the building blocks may vary considerably, the forces that drive their formation, the local interactions, the structure and the morphology are closely related. Cryo-EM methodologies (Cryo-TEM in the colloid science community) have proven powerful in resolving structure-function-property relations of these macromolecular assemblies. The present review contains two main sections highlighting the unique cryo-EM contribution to understanding the global and the local features of nano-fibers, ribbons and tubes, as well as their detailed high-resolution structure.
KW - Cryo-EM
KW - Cryo-TEM
KW - Helical reconstruction
KW - Image processing
KW - Ribbons and nanotubes
UR - http://www.scopus.com/inward/record.url?scp=85048727599&partnerID=8YFLogxK
U2 - 10.1016/j.cocis.2018.05.002
DO - 10.1016/j.cocis.2018.05.002
M3 - 文献综述
AN - SCOPUS:85048727599
SN - 1359-0294
VL - 34
SP - 100
EP - 113
JO - Current Opinion in Colloid and Interface Science
JF - Current Opinion in Colloid and Interface Science
ER -