Differential distribution patterns of ammonia-oxidizing archaea and bacteria in acidic soils of Nanling National Nature Reserve forests in subtropical China

TY - JOUR

T1 - Differential distribution patterns of ammonia-oxidizing archaea and bacteria in acidic soils of Nanling National Nature Reserve forests in subtropical China

AU - Gan, Xian Hua

AU - Zhang, Fang Qiu

AU - Gu, Ji Dong

AU - Guo, Yue Dong

AU - Li, Zhao Qing

AU - Zhang, Wei Qiang

AU - Xu, Xiu Yu

AU - Zhou, Yi

AU - Wen, Xiao Ying

AU - Xie, Guo Guang

AU - Wang, Yong Feng

N1 - Publisher Copyright: © 2015, Springer International Publishing Switzerland.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - In addition to ammonia-oxidizing bacteria (AOB) the more recently discovered ammonia-oxidizing archaea (AOA) can also oxidize ammonia, but little is known about AOA community structure and abundance in subtropical forest soils. In this study, both AOA and AOB were investigated with molecular techniques in eight types of forests at surface soils (0–2 cm) and deep layers (18–20 cm) in Nanling National Nature Reserve in subtropical China. The results showed that the forest soils, all acidic (pH 4.24–5.10), harbored a wide range of AOA phylotypes, including the genera Nitrosotalea, Nitrososphaera, and another 6 clusters, one of which was reported for the first time. For AOB, only members of Nitrosospira were retrieved. Moreover, the abundance of the ammonia monooxygenase gene (amoA) from AOA dominated over AOB in most soil samples (13/16). Soil depth, rather than forest type, was an important factor shaping the community structure of AOA and AOB. The distribution patterns of AOA and AOB in soil layers were reversed: AOA diversity and abundances in the deep layers were higher than those in the surface layers; on the contrary, AOB diversity and abundances in the deep layers were lower than those in the surface layers. Interestingly, the diversity of AOA was positively correlated with pH, but negatively correlated with organic carbon, total nitrogen and total phosphorus, and the abundance of AOA was negatively correlated with available phosphorus. Our results demonstrated that AOA and AOB were differentially distributed in acidic soils in subtropical forests and affected differently by soil characteristics.

AB - In addition to ammonia-oxidizing bacteria (AOB) the more recently discovered ammonia-oxidizing archaea (AOA) can also oxidize ammonia, but little is known about AOA community structure and abundance in subtropical forest soils. In this study, both AOA and AOB were investigated with molecular techniques in eight types of forests at surface soils (0–2 cm) and deep layers (18–20 cm) in Nanling National Nature Reserve in subtropical China. The results showed that the forest soils, all acidic (pH 4.24–5.10), harbored a wide range of AOA phylotypes, including the genera Nitrosotalea, Nitrososphaera, and another 6 clusters, one of which was reported for the first time. For AOB, only members of Nitrosospira were retrieved. Moreover, the abundance of the ammonia monooxygenase gene (amoA) from AOA dominated over AOB in most soil samples (13/16). Soil depth, rather than forest type, was an important factor shaping the community structure of AOA and AOB. The distribution patterns of AOA and AOB in soil layers were reversed: AOA diversity and abundances in the deep layers were higher than those in the surface layers; on the contrary, AOB diversity and abundances in the deep layers were lower than those in the surface layers. Interestingly, the diversity of AOA was positively correlated with pH, but negatively correlated with organic carbon, total nitrogen and total phosphorus, and the abundance of AOA was negatively correlated with available phosphorus. Our results demonstrated that AOA and AOB were differentially distributed in acidic soils in subtropical forests and affected differently by soil characteristics.

KW - Acidic soil

KW - Ammonia-oxidizing archaea

KW - Nanling Mountains

KW - Subtropical forest

KW - Thaumarchaeota

UR - http://www.scopus.com/inward/record.url?scp=84957963582&partnerID=8YFLogxK

U2 - 10.1007/s10482-015-0627-8

DO - 10.1007/s10482-015-0627-8

M3 - 文章

C2 - 26626057

AN - SCOPUS:84957963582

SN - 0003-6072

VL - 109

SP - 237

EP - 251

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

IS - 2

ER -