Different diversity and distribution of archaeal community in the aqueous and oil phases of production fluid from high-temperature petroleum reservoirs

TY - JOUR

T1 - Different diversity and distribution of archaeal community in the aqueous and oil phases of production fluid from high-temperature petroleum reservoirs

AU - Liang, Bo

AU - Zhang, Kai

AU - Wang, Li Ying

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Publisher Copyright: © 2018 Liang, Zhang, Wang, Liu, Yang, Gu and Mu.

PY - 2018/4/27

Y1 - 2018/4/27

N2 - To get a better knowledge on how archaeal communities differ between the oil and aqueous phases and whether environmental factors promote substantial differences on microbial distributions among production wells, we analyzed archaeal communities in oil and aqueous phases from four high-temperature petroleum reservoirs (55-65°C) by using 16S rRNA gene based 454 pyrosequencing. Obvious dissimilarity of the archaeal composition between aqueous and oil phases in each independent production wells was observed, especially in production wells with higher water cut, and diversity in the oil phase was much higher than that in the corresponding aqueous phase. Statistical analysis further showed that archaeal communities in oil phases from different petroleum reservoirs tended to be more similar, but those in aqueous phases were the opposite. In the high-temperature ecosystems, temperature as an environmental factor could have significantly affected archaeal distribution, and archaeal diversity raised with the increase of temperature (p < 0.05). Our results suggest that to get a comprehensive understanding of petroleum reservoirs microbial information both in aqueous and oil phases should be taken into consideration. The microscopic habitats of oil phase, technically the dispersed minuscule water droplets in the oil could be a better habitat that containing the indigenous microorganisms.

AB - To get a better knowledge on how archaeal communities differ between the oil and aqueous phases and whether environmental factors promote substantial differences on microbial distributions among production wells, we analyzed archaeal communities in oil and aqueous phases from four high-temperature petroleum reservoirs (55-65°C) by using 16S rRNA gene based 454 pyrosequencing. Obvious dissimilarity of the archaeal composition between aqueous and oil phases in each independent production wells was observed, especially in production wells with higher water cut, and diversity in the oil phase was much higher than that in the corresponding aqueous phase. Statistical analysis further showed that archaeal communities in oil phases from different petroleum reservoirs tended to be more similar, but those in aqueous phases were the opposite. In the high-temperature ecosystems, temperature as an environmental factor could have significantly affected archaeal distribution, and archaeal diversity raised with the increase of temperature (p < 0.05). Our results suggest that to get a comprehensive understanding of petroleum reservoirs microbial information both in aqueous and oil phases should be taken into consideration. The microscopic habitats of oil phase, technically the dispersed minuscule water droplets in the oil could be a better habitat that containing the indigenous microorganisms.

KW - Aqueous phase

KW - Archaeal community

KW - Exogenous microorganisms

KW - Indigenous microorganisms

KW - Oil phase

KW - Petroleum reservoir

KW - Water flooding

UR - http://www.scopus.com/inward/record.url?scp=85046088793&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2018.00841

DO - 10.3389/fmicb.2018.00841

M3 - 文章

AN - SCOPUS:85046088793

SN - 1664-302X

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

IS - APR

M1 - 841

ER -