Differences in intramembrane particle distribution in young and old human erythrocytes

TY - JOUR

T1 - Differences in intramembrane particle distribution in young and old human erythrocytes

AU - Cordero, Julio F.

AU - Rodríguez, Pedro J.

AU - Romero, Pedro J.

N1 - Funding Information: This work was financially supported by Consejo de Desarrollo Cientı́fico y Humanı́stico of Universidad Central de Venezuela, CDCH-UCV (Grant 03.10.3836.1999) and Consejo Nacional de Investigaciones Cientı́ficas, CONICIT (Grant S1-97000369). Dr. Cristian Puig from Polymers Laboratory, Universidad Simón Bolı́var is especially acknowledged for lending the electron microscopy facilities. We would also like to acknowledge Mr. Diego Rojas for his excellent technical assistance.

PY - 2004/6

Y1 - 2004/6

N2 - The distribution of intramembrane particles in human erythrocytes was studied by freeze-fracture on young and old cells and compared to that obtained after ATP depletion or following addition of a clustering agent. It was shown that intramembrane particles became aggregated and the mean particle density increased as the cells aged. Likewise, both particle aggregation and increased density were found in young cells after moderate ATP depletion. In contrast, mean particle density was markedly reduced in both cell types after exhaustive depletion. Paradoxically, Zn treatment led to decreased particle density in young cells, whilst producing the opposite effect in aged cells. The results suggest that their low ATP content may account for the increased particle density of senescent cells.

AB - The distribution of intramembrane particles in human erythrocytes was studied by freeze-fracture on young and old cells and compared to that obtained after ATP depletion or following addition of a clustering agent. It was shown that intramembrane particles became aggregated and the mean particle density increased as the cells aged. Likewise, both particle aggregation and increased density were found in young cells after moderate ATP depletion. In contrast, mean particle density was markedly reduced in both cell types after exhaustive depletion. Paradoxically, Zn treatment led to decreased particle density in young cells, whilst producing the opposite effect in aged cells. The results suggest that their low ATP content may account for the increased particle density of senescent cells.

KW - Ageing

KW - Freeze-fracture

KW - Human erythrocytes

KW - IMP

KW - Red cell sub-populations

UR - http://www.scopus.com/inward/record.url?scp=3042637830&partnerID=8YFLogxK

U2 - 10.1016/j.cellbi.2004.03.002

DO - 10.1016/j.cellbi.2004.03.002

M3 - 文章

C2 - 15223018

AN - SCOPUS:3042637830

SN - 1065-6995

VL - 28

SP - 423

EP - 431

JO - Cell Biology International

JF - Cell Biology International

IS - 6

ER -