TY - JOUR
T1 - Degradation of p-nitrophenol by Achromobacter xylosoxidans Ns isolated from wetland sediment
AU - Wan, Niansheng
AU - Gu, Ji Dong
AU - Yan, Yan
N1 - Funding Information:
This research was supported by Research Grant no. ZKCX2-SW-212-4 of the Chinese Academy of Sciences, and SCSIO Project no. LYQY200306. We thank Pan Li for the determination of the 16S rDNA sequences.
PY - 2007/3
Y1 - 2007/3
N2 - Achromobacter xylosoxidans Ns strain, capable of utilizing p-nitrophenol (PNP) as the sole source of carbon, energy, and nitrogen, was isolated from wetland sediment and confirmed based on 16S rRNA gene sequence. The strain Ns could tolerate concentrations of PNP up to 1.8 mM, and degradation of PNP was achieved in 7 d at 30 °C in the dark under aerobic conditions. Biodegradation of PNP occurred quickly at an optimal pH of 7.0 and higher, and at ≤0.5% salt (NaCl) contents. During bacterial growth on PNP, 4-nitrocatechol was observed as a key degradation intermediate using a combination of techniques, including HPLC, UV-visible spectra, and comparison with the authentic standard. In a similar way, a second degradation intermediate was identified to be 1,2,4-benzenetriol. Moreover, A. xylosoxidans Ns could also degrade 3-nitrophenol as the sole source of carbon, nitrogen, and energy, but 2-nitrophenol could not. The experimental results showed that bacteria indigenous to the wetland sediment are capable of degradading PNP and chemicals with similar structures.
AB - Achromobacter xylosoxidans Ns strain, capable of utilizing p-nitrophenol (PNP) as the sole source of carbon, energy, and nitrogen, was isolated from wetland sediment and confirmed based on 16S rRNA gene sequence. The strain Ns could tolerate concentrations of PNP up to 1.8 mM, and degradation of PNP was achieved in 7 d at 30 °C in the dark under aerobic conditions. Biodegradation of PNP occurred quickly at an optimal pH of 7.0 and higher, and at ≤0.5% salt (NaCl) contents. During bacterial growth on PNP, 4-nitrocatechol was observed as a key degradation intermediate using a combination of techniques, including HPLC, UV-visible spectra, and comparison with the authentic standard. In a similar way, a second degradation intermediate was identified to be 1,2,4-benzenetriol. Moreover, A. xylosoxidans Ns could also degrade 3-nitrophenol as the sole source of carbon, nitrogen, and energy, but 2-nitrophenol could not. The experimental results showed that bacteria indigenous to the wetland sediment are capable of degradading PNP and chemicals with similar structures.
KW - 3-nitrophenol
KW - Achromobacter xylosoxidans
KW - Biodegradation
KW - Wetland
KW - p-nitrophenol
UR - http://www.scopus.com/inward/record.url?scp=33846559758&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2006.07.012
DO - 10.1016/j.ibiod.2006.07.012
M3 - 文章
AN - SCOPUS:33846559758
SN - 0964-8305
VL - 59
SP - 90
EP - 96
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 2
ER -