Cryo-TEM and NMR studies of a micelle-forming phosphoglucolipid from membranes of Acholeplasma laidlawii A and B

TY - JOUR

T1 - Cryo-TEM and NMR studies of a micelle-forming phosphoglucolipid from membranes of Acholeplasma laidlawii A and B

AU - Danino, Dganit

AU - Kaplun, Alon

AU - Lindblom, Göran

AU - Rilfors, Leif

AU - Orädd, Greger

AU - Hauksson, Jón B.

AU - Talmon, Yeshayahu

N1 - Funding Information: We thank The Swedish Natural Science Research Council and The Knut and Alice Wallenberg Foundation for financial support. GPDGlcDAG from strain A. laidlawii B-PG9 was kindly provided by Drs R.N.A.H. Lewis and R.N. McElhaney at the Department of Biochemistry, University of Alberta, Edmonton, Canada. Susann Haraldsson is acknowledged for performing the gas chromatography analyses of the acyl chain compositions.

PY - 1997/1/17

Y1 - 1997/1/17

N2 - The chemical structure of a phosphoglucolipid from the membrane of the bacterium Acholeplasma laidlawii strain B-PG9 has been determined by high resolution NMR to be 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α-D-glucopyranosyl-(1 → 2)-O-α-D-glucopyranosyl)]-sn-glycerol (GPDGlcDAG). It was concluded that this lipid has exactly the same structure as one of the phosphoglucolipids from A. laidlawii strain A-EF22. By cryo transmission electron microscopy (cryo-TEM) and NMR diffusion techniques it was shown that, in highly diluted aqueous solutions, this membrane lipid forms long thread-like micelles in equilibrium with lipid vesicles. The cause of the occurrence of these different aggregates is discussed in terms of the varying molecular shapes of the lipid because of a heterogeneous composition of the acyl chains. A second membrane phosphoglucolipid from the bacterium, namely 1,2-diacyl-3-O[glycerophosphoryl-6-O-(α-D-glucopyranosyl-(1 → 2)-monoacylglycerophosphoryl-6-O-α-D-glucopyranosyl)]-sn-glycerol (MABGPDGlcDAG), was found to form only a lamellar liquid crystalline phase coexisting with water.

AB - The chemical structure of a phosphoglucolipid from the membrane of the bacterium Acholeplasma laidlawii strain B-PG9 has been determined by high resolution NMR to be 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(α-D-glucopyranosyl-(1 → 2)-O-α-D-glucopyranosyl)]-sn-glycerol (GPDGlcDAG). It was concluded that this lipid has exactly the same structure as one of the phosphoglucolipids from A. laidlawii strain A-EF22. By cryo transmission electron microscopy (cryo-TEM) and NMR diffusion techniques it was shown that, in highly diluted aqueous solutions, this membrane lipid forms long thread-like micelles in equilibrium with lipid vesicles. The cause of the occurrence of these different aggregates is discussed in terms of the varying molecular shapes of the lipid because of a heterogeneous composition of the acyl chains. A second membrane phosphoglucolipid from the bacterium, namely 1,2-diacyl-3-O[glycerophosphoryl-6-O-(α-D-glucopyranosyl-(1 → 2)-monoacylglycerophosphoryl-6-O-α-D-glucopyranosyl)]-sn-glycerol (MABGPDGlcDAG), was found to form only a lamellar liquid crystalline phase coexisting with water.

KW - Acholeplasma laidlawii

KW - Cryo-TEM

KW - Glycerophosphoryldiglucosyldiacylglycerol

KW - Lipid structure

KW - Micelle

KW - NMR

UR - http://www.scopus.com/inward/record.url?scp=0031014237&partnerID=8YFLogxK

U2 - 10.1016/S0009-3084(96)02640-0

DO - 10.1016/S0009-3084(96)02640-0

M3 - 文章

C2 - 9032946

AN - SCOPUS:0031014237

SN - 0009-3084

VL - 85

SP - 75

EP - 89

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

IS - 1

ER -