TY - JOUR
T1 - Complete degradation of dimethyl isophthalate requires the biochemical cooperation between Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr Isolated from Wetland sediment
AU - Li, Jiaxi
AU - Gu, Ji Dong
N1 - Funding Information:
This project was supported by the Chinese Academy of Sciences and additional financial support was by a Knowledge Innovation project of SCSIO (No. LYQY200306) and an 863 project (No. 2002AA601160).
PY - 2007/7/15
Y1 - 2007/7/15
N2 - Two bacterial strains Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr were isolated and identified from enrichment cultures using dimethyl isophthalate (DMI) as the sole source of carbon and energy, and mangrove sediment as an inoculum. DMI was rapidly transformed by K. oxytoca Sc in the culture with formation of monomethyl isophthalate (MMI), which accumulated in the culture medium. M. mesophilicum Sr, incapable of utilizing DMI, showed high capability of degrading MMI to a transitory intermediate isophthalic acid (IPA), which was further mineralized by this strain. The biochemical pathway of DMI degradation by these two bacteria in a consortium was proposed: DMI to MMI by K. oxytoca Sc, MMI to IPA by M. mesophilicum Sr, and IPA by both K. oxytoca Sc and M. mesophilicum Sr based on the identified degradation intermediates. The consortium comprising K. oxytoca Sc and M. mesophilicum Sr was effective in mineralization of DMI. The results suggest that complete degradation of environmental pollutant DMI requires the biochemical cooperation between different microorganisms of the mangrove environment.
AB - Two bacterial strains Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr were isolated and identified from enrichment cultures using dimethyl isophthalate (DMI) as the sole source of carbon and energy, and mangrove sediment as an inoculum. DMI was rapidly transformed by K. oxytoca Sc in the culture with formation of monomethyl isophthalate (MMI), which accumulated in the culture medium. M. mesophilicum Sr, incapable of utilizing DMI, showed high capability of degrading MMI to a transitory intermediate isophthalic acid (IPA), which was further mineralized by this strain. The biochemical pathway of DMI degradation by these two bacteria in a consortium was proposed: DMI to MMI by K. oxytoca Sc, MMI to IPA by M. mesophilicum Sr, and IPA by both K. oxytoca Sc and M. mesophilicum Sr based on the identified degradation intermediates. The consortium comprising K. oxytoca Sc and M. mesophilicum Sr was effective in mineralization of DMI. The results suggest that complete degradation of environmental pollutant DMI requires the biochemical cooperation between different microorganisms of the mangrove environment.
KW - Biochemical cooperation
KW - Biodegradation
KW - Dimethyl isophthalate
KW - Isophthalic acid
KW - Monomethyl isophthalate
KW - Plasticizers
UR - http://www.scopus.com/inward/record.url?scp=34249096443&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2006.12.033
DO - 10.1016/j.scitotenv.2006.12.033
M3 - 文章
C2 - 17258288
AN - SCOPUS:34249096443
SN - 0048-9697
VL - 380
SP - 181
EP - 187
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 1-3
ER -