TY - JOUR
T1 - Community structure and abundance of ammonia-oxidizing archaea and bacteria after conversion from soybean to rice paddy in albic soils of Northeast China
AU - Wang, Jing
AU - Wang, Weidong
AU - Gu, Ji Dong
N1 - Funding Information:
Acknowledgments The authors would like to thank The University of Hong Kong for the award of a postgraduate scholarship (JW). This project was supported by the RGC GRF grant no. 701913 (J-DG) and the additional research fund of Environmental Toxicology Education of this laboratory. The help and assistance of Mr. Dehui Shi from Honghe State Farm for information about the crop cultivation and land use history and sampling on site and the logistic transportation arrangement by Ms. Zheng Chunyu for the samplings carried out are both acknowledged. Laboratory assistance was provided by Jessie Lai at The University of Hong Kong.
PY - 2014/3
Y1 - 2014/3
N2 - Community composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in the albic soil grown with soybean and rice for different years was investigated by construction of clone libraries, denaturing gradient gel electrophoresis (DGGE), and quantitative polymerase chain reaction (q-PCR) by PCR amplification of the ammonia monooxygenase subunit A (amoA) gene. Soil samples were collected at two layers (0-5 and 20-25 cm) from a soybean field and four rice paddy fields with 1, 5, 9, and 17 years of continuous rice cultivation. Both the community structures and abundances of AOA and AOB showed detectable changes after conversion from soybean to rice paddy judged by clone library, DGGE, and q-PCR analyses. In general, the archaeal amoA gene abundance increased after conversion to rice cultivation, while bacterial amoA gene abundance decreased. The abundances of both AOA and AOB were higher in the surface layer than the bottom one in the soybean field, but a reverse trend was observed for AOB in all paddy samples regardless of the duration of paddy cultivation. Phylogenetic analysis identified nine subclusters of AOA and seven subclusters of AOB. Community composition of both AOA and AOB was correlated with available ammonium and increased pH value caused by flooding in multiple variance analysis. Community shift of AOB was also observed in different paddy fields, but the two layers did not show any detectable changes in DGGE analysis. Conversion from soybean to rice cultivation changed the community structure and abundance of AOA and AOB in albic agricultural soil, which requires that necessary cultivation practice be followed to manage the N utilization more effectively.
AB - Community composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in the albic soil grown with soybean and rice for different years was investigated by construction of clone libraries, denaturing gradient gel electrophoresis (DGGE), and quantitative polymerase chain reaction (q-PCR) by PCR amplification of the ammonia monooxygenase subunit A (amoA) gene. Soil samples were collected at two layers (0-5 and 20-25 cm) from a soybean field and four rice paddy fields with 1, 5, 9, and 17 years of continuous rice cultivation. Both the community structures and abundances of AOA and AOB showed detectable changes after conversion from soybean to rice paddy judged by clone library, DGGE, and q-PCR analyses. In general, the archaeal amoA gene abundance increased after conversion to rice cultivation, while bacterial amoA gene abundance decreased. The abundances of both AOA and AOB were higher in the surface layer than the bottom one in the soybean field, but a reverse trend was observed for AOB in all paddy samples regardless of the duration of paddy cultivation. Phylogenetic analysis identified nine subclusters of AOA and seven subclusters of AOB. Community composition of both AOA and AOB was correlated with available ammonium and increased pH value caused by flooding in multiple variance analysis. Community shift of AOB was also observed in different paddy fields, but the two layers did not show any detectable changes in DGGE analysis. Conversion from soybean to rice cultivation changed the community structure and abundance of AOA and AOB in albic agricultural soil, which requires that necessary cultivation practice be followed to manage the N utilization more effectively.
KW - Albic soil
KW - Ammonia monooxygenase subunit A (amoA)
KW - Ammonia-oxidizing archaea
KW - Ammonia-oxidizing bacteria
KW - Baijiang soil
KW - Paddy
KW - Sangjiang Plain
UR - http://www.scopus.com/inward/record.url?scp=84896827245&partnerID=8YFLogxK
U2 - 10.1007/s00253-013-5213-2
DO - 10.1007/s00253-013-5213-2
M3 - 文章
C2 - 24092004
AN - SCOPUS:84896827245
SN - 0175-7598
VL - 98
SP - 2765
EP - 2778
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 6
ER -