Biogeographic pattern of the nirS gene-targeted anammox bacterial community and composition in the northern South China Sea and a coastal Mai Po mangrove wetland

Zhichao Zhou; Jing Chen; Wenjie Gu; Ji Dong Gu

doi:10.1007/s00253-020-10415-3

Biogeographic pattern of the nirS gene-targeted anammox bacterial community and composition in the northern South China Sea and a coastal Mai Po mangrove wetland

Zhichao Zhou, Jing Chen, Wenjie Gu, Ji Dong Gu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Functional genes, namely hzo/hao, nirS, hzs, and ccs gene, are efficient with high specificity for detecting anammox bacteria. Sc-nirS and An-nirS primer sets were proposed for targeting Scalindua/non-Scalindua anammox bacterial groups; previously, they have not been assessed for biogeographic study on marine-terrestrial transitional systems, specifically marine and terrestrial ecosystems. Here, we report phylogenetic distribution pattern of anammox bacteria in both northern South China Sea (nSCS) and Mai Po wetland (a coastal mangrove) using nirS gene-based primers. A well-delineated biogeographic distribution pattern from ocean to continental shelf was evident by combining both gene-based analyses as previously depicted using 16S rRNA as the biomarker. Furthermore, factors affecting the abundance and composition of An-nirS genes in Mai Po wetland were identified as substrate (NO₃ ⁻/NO₂ ⁻ concentration) and anoxic/oxic condition in association to depth. An-nirS gene abundance was from 2.6 × 10³ to 1.2–1.4 × 10⁴ copies/g dry sediment in nSCS; and it was around 5 × 10³ and 1–2 × 10⁴ copies/g dry sediment in surface and subsurface sediments of Mai Po wetland, respectively. In addition, nirS gene abundance and distribution pattern of denitrifiers and anammox bacteria in the wetland indicates a competition relationship between them. Mangrove vegetation affected the community composition of An-nirS gene considerably, and a more homogeneous distribution pattern was observed in the mangrove forest than intertidal mudflats. Sc/An-nirS gene-based biogeographic insights on anammox bacteria have shed lights on the compositional and potential functional dynamics and emphasized the importance of molecular tools on refining the current microbial ecological patterns.

Original language	English
Pages (from-to)	3167-3181
Number of pages	15
Journal	Applied Microbiology and Biotechnology
Volume	104
Issue number	7
DOIs	https://doi.org/10.1007/s00253-020-10415-3
State	Published - 1 Apr 2020
Externally published	Yes

Keywords

Anammox
Biogeographic distribution
Mai Po wetland
Marine-terrestrial transition
Northern South China Sea
nirS gene

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00253-020-10415-3

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title = "Biogeographic pattern of the nirS gene-targeted anammox bacterial community and composition in the northern South China Sea and a coastal Mai Po mangrove wetland",

abstract = "Functional genes, namely hzo/hao, nirS, hzs, and ccs gene, are efficient with high specificity for detecting anammox bacteria. Sc-nirS and An-nirS primer sets were proposed for targeting Scalindua/non-Scalindua anammox bacterial groups; previously, they have not been assessed for biogeographic study on marine-terrestrial transitional systems, specifically marine and terrestrial ecosystems. Here, we report phylogenetic distribution pattern of anammox bacteria in both northern South China Sea (nSCS) and Mai Po wetland (a coastal mangrove) using nirS gene-based primers. A well-delineated biogeographic distribution pattern from ocean to continental shelf was evident by combining both gene-based analyses as previously depicted using 16S rRNA as the biomarker. Furthermore, factors affecting the abundance and composition of An-nirS genes in Mai Po wetland were identified as substrate (NO3 −/NO2 − concentration) and anoxic/oxic condition in association to depth. An-nirS gene abundance was from 2.6 × 103 to 1.2–1.4 × 104 copies/g dry sediment in nSCS; and it was around 5 × 103 and 1–2 × 104 copies/g dry sediment in surface and subsurface sediments of Mai Po wetland, respectively. In addition, nirS gene abundance and distribution pattern of denitrifiers and anammox bacteria in the wetland indicates a competition relationship between them. Mangrove vegetation affected the community composition of An-nirS gene considerably, and a more homogeneous distribution pattern was observed in the mangrove forest than intertidal mudflats. Sc/An-nirS gene-based biogeographic insights on anammox bacteria have shed lights on the compositional and potential functional dynamics and emphasized the importance of molecular tools on refining the current microbial ecological patterns.",

keywords = "Anammox, Biogeographic distribution, Mai Po wetland, Marine-terrestrial transition, Northern South China Sea, nirS gene",

author = "Zhichao Zhou and Jing Chen and Wenjie Gu and Gu, {Ji Dong}",

note = "Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2020",

month = apr,

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doi = "10.1007/s00253-020-10415-3",

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T1 - Biogeographic pattern of the nirS gene-targeted anammox bacterial community and composition in the northern South China Sea and a coastal Mai Po mangrove wetland

AU - Zhou, Zhichao

AU - Chen, Jing

AU - Gu, Wenjie

AU - Gu, Ji Dong

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Functional genes, namely hzo/hao, nirS, hzs, and ccs gene, are efficient with high specificity for detecting anammox bacteria. Sc-nirS and An-nirS primer sets were proposed for targeting Scalindua/non-Scalindua anammox bacterial groups; previously, they have not been assessed for biogeographic study on marine-terrestrial transitional systems, specifically marine and terrestrial ecosystems. Here, we report phylogenetic distribution pattern of anammox bacteria in both northern South China Sea (nSCS) and Mai Po wetland (a coastal mangrove) using nirS gene-based primers. A well-delineated biogeographic distribution pattern from ocean to continental shelf was evident by combining both gene-based analyses as previously depicted using 16S rRNA as the biomarker. Furthermore, factors affecting the abundance and composition of An-nirS genes in Mai Po wetland were identified as substrate (NO3 −/NO2 − concentration) and anoxic/oxic condition in association to depth. An-nirS gene abundance was from 2.6 × 103 to 1.2–1.4 × 104 copies/g dry sediment in nSCS; and it was around 5 × 103 and 1–2 × 104 copies/g dry sediment in surface and subsurface sediments of Mai Po wetland, respectively. In addition, nirS gene abundance and distribution pattern of denitrifiers and anammox bacteria in the wetland indicates a competition relationship between them. Mangrove vegetation affected the community composition of An-nirS gene considerably, and a more homogeneous distribution pattern was observed in the mangrove forest than intertidal mudflats. Sc/An-nirS gene-based biogeographic insights on anammox bacteria have shed lights on the compositional and potential functional dynamics and emphasized the importance of molecular tools on refining the current microbial ecological patterns.

AB - Functional genes, namely hzo/hao, nirS, hzs, and ccs gene, are efficient with high specificity for detecting anammox bacteria. Sc-nirS and An-nirS primer sets were proposed for targeting Scalindua/non-Scalindua anammox bacterial groups; previously, they have not been assessed for biogeographic study on marine-terrestrial transitional systems, specifically marine and terrestrial ecosystems. Here, we report phylogenetic distribution pattern of anammox bacteria in both northern South China Sea (nSCS) and Mai Po wetland (a coastal mangrove) using nirS gene-based primers. A well-delineated biogeographic distribution pattern from ocean to continental shelf was evident by combining both gene-based analyses as previously depicted using 16S rRNA as the biomarker. Furthermore, factors affecting the abundance and composition of An-nirS genes in Mai Po wetland were identified as substrate (NO3 −/NO2 − concentration) and anoxic/oxic condition in association to depth. An-nirS gene abundance was from 2.6 × 103 to 1.2–1.4 × 104 copies/g dry sediment in nSCS; and it was around 5 × 103 and 1–2 × 104 copies/g dry sediment in surface and subsurface sediments of Mai Po wetland, respectively. In addition, nirS gene abundance and distribution pattern of denitrifiers and anammox bacteria in the wetland indicates a competition relationship between them. Mangrove vegetation affected the community composition of An-nirS gene considerably, and a more homogeneous distribution pattern was observed in the mangrove forest than intertidal mudflats. Sc/An-nirS gene-based biogeographic insights on anammox bacteria have shed lights on the compositional and potential functional dynamics and emphasized the importance of molecular tools on refining the current microbial ecological patterns.

KW - Anammox

KW - Biogeographic distribution

KW - Mai Po wetland

KW - Marine-terrestrial transition

KW - Northern South China Sea

KW - nirS gene

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U2 - 10.1007/s00253-020-10415-3

DO - 10.1007/s00253-020-10415-3

M3 - 文章

C2 - 32036435

AN - SCOPUS:85079468496

SN - 0175-7598

VL - 104

SP - 3167

EP - 3181

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 7

ER -

Biogeographic pattern of the nirS gene-targeted anammox bacterial community and composition in the northern South China Sea and a coastal Mai Po mangrove wetland

Abstract

Keywords

UN SDGs

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