Biocatalytic implant of Pt nanoclusters into glucose oxidase: A method to electrically wire the enzyme and to transform it from an oxidase to a hydrogenase

Omer Yehezkeli; Sara Raichlin; Ran Tel-Vered; Ellina Kesselman; Dganit Danino; Itamar Willner

doi:10.1021/jz1011324

Biocatalytic implant of Pt nanoclusters into glucose oxidase: A method to electrically wire the enzyme and to transform it from an oxidase to a hydrogenase

Omer Yehezkeli, Sara Raichlin, Ran Tel-Vered, Ellina Kesselman, Dganit Danino, Itamar Willner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The enzyme glucose oxidase (GOx) reduces, in the presence of glucose and under anaerobic conditions, PtCl₆^2- to Pt nanoclusters (NCs) that are implanted in the protein. The assembly of the Pt NC/GOx hybrid on a dithiol monolayer yields an electrically contacted enzyme electrode, and the bioelectrocatalytic oxidation of glucose is activated (turnover rate ca. 2780 ± 70 s^-1). The Pt NC/GOx hybrid is also used, under anaerobic conditions, as a biocatalyst for H₂ evolution.

Original language	English
Pages (from-to)	2816-2819
Number of pages	4
Journal	Journal of Physical Chemistry Letters
Volume	1
Issue number	19
DOIs	https://doi.org/10.1021/jz1011324
State	Published - 7 Oct 2010
Externally published	Yes

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title = "Biocatalytic implant of Pt nanoclusters into glucose oxidase: A method to electrically wire the enzyme and to transform it from an oxidase to a hydrogenase",

abstract = "The enzyme glucose oxidase (GOx) reduces, in the presence of glucose and under anaerobic conditions, PtCl62- to Pt nanoclusters (NCs) that are implanted in the protein. The assembly of the Pt NC/GOx hybrid on a dithiol monolayer yields an electrically contacted enzyme electrode, and the bioelectrocatalytic oxidation of glucose is activated (turnover rate ca. 2780 ± 70 s-1). The Pt NC/GOx hybrid is also used, under anaerobic conditions, as a biocatalyst for H2 evolution.",

author = "Omer Yehezkeli and Sara Raichlin and Ran Tel-Vered and Ellina Kesselman and Dganit Danino and Itamar Willner",

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doi = "10.1021/jz1011324",

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T2 - A method to electrically wire the enzyme and to transform it from an oxidase to a hydrogenase

AU - Yehezkeli, Omer

AU - Raichlin, Sara

AU - Tel-Vered, Ran

AU - Kesselman, Ellina

AU - Danino, Dganit

AU - Willner, Itamar

PY - 2010/10/7

Y1 - 2010/10/7

N2 - The enzyme glucose oxidase (GOx) reduces, in the presence of glucose and under anaerobic conditions, PtCl62- to Pt nanoclusters (NCs) that are implanted in the protein. The assembly of the Pt NC/GOx hybrid on a dithiol monolayer yields an electrically contacted enzyme electrode, and the bioelectrocatalytic oxidation of glucose is activated (turnover rate ca. 2780 ± 70 s-1). The Pt NC/GOx hybrid is also used, under anaerobic conditions, as a biocatalyst for H2 evolution.

AB - The enzyme glucose oxidase (GOx) reduces, in the presence of glucose and under anaerobic conditions, PtCl62- to Pt nanoclusters (NCs) that are implanted in the protein. The assembly of the Pt NC/GOx hybrid on a dithiol monolayer yields an electrically contacted enzyme electrode, and the bioelectrocatalytic oxidation of glucose is activated (turnover rate ca. 2780 ± 70 s-1). The Pt NC/GOx hybrid is also used, under anaerobic conditions, as a biocatalyst for H2 evolution.

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U2 - 10.1021/jz1011324

DO - 10.1021/jz1011324

M3 - 文章

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VL - 1

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JO - Journal of Physical Chemistry Letters

JF - Journal of Physical Chemistry Letters

IS - 19

ER -

Biocatalytic implant of Pt nanoclusters into glucose oxidase: A method to electrically wire the enzyme and to transform it from an oxidase to a hydrogenase

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