TY - JOUR
T1 - Aminirod propionatiphilus gen. nov., sp. nov., an isolated secondary fermenter in methanogenic hydrocarbon-degrading communities
AU - Liu, Yi Fan
AU - Liu, Zhong Lin
AU - Ye, Yang Li
AU - Zhou, Lei
AU - Liu, Jin Feng
AU - Yang, Shi Zhong
AU - Gu, Ji Dong
AU - Mu, Bo Zhong
N1 - Publisher Copyright:
© 2021
PY - 2021/11
Y1 - 2021/11
N2 - During microbial methanogenic hydrocarbon degradation, secondary fermenters that convert the substrates produced by primary fermenters to acetate, carbon dioxide, hydrogen, and formate which could be further transformed into methane by terminal methanogens have received less attention. In this study, we isolated an anaerobic bacterium, designated strain L-13T, from a methanogenic crude oil-degrading enrichment culture. The motile, non-sporulating, tenuous rod cells (0.5–0.8 μm) stained Gram-positive and grew optimally at 37 °C and pH 7.2 with 0.5% NaCl. 16S rRNA gene sequence analysis revealed that strain L-13T was distantly related to members of the genus Aminiphilus (89% similarity) in the family Aminiphilaceae of the phylum Synergistetes, and represented a novel genus-level clade, previously known as EBM-39. Fermentative growth with various amino acids, propionate and some specific sugars was observed, with acetate and hydrogen as the main end products. Syntrophic growth occurred with propionate, butyrate and caprylate in the presence of an H2-utilizing methanogen, and growth on butyrate and caprylate was only observed under syntrophic conditions. Therefore, this strain may play roles as both secondary fermenter and biomass scavenger in the engineered anaerobic bioreactors/digestors and laboratory enrichments, where primary fermentation products and necromass are readily available. Based on chemotaxonomic, phylogenetic and genomic characteristics, strain L-13T (=CGMCC 1.17908) was identified and nominated as Aminirod propionatiphilus gen. nov., sp. nov.
AB - During microbial methanogenic hydrocarbon degradation, secondary fermenters that convert the substrates produced by primary fermenters to acetate, carbon dioxide, hydrogen, and formate which could be further transformed into methane by terminal methanogens have received less attention. In this study, we isolated an anaerobic bacterium, designated strain L-13T, from a methanogenic crude oil-degrading enrichment culture. The motile, non-sporulating, tenuous rod cells (0.5–0.8 μm) stained Gram-positive and grew optimally at 37 °C and pH 7.2 with 0.5% NaCl. 16S rRNA gene sequence analysis revealed that strain L-13T was distantly related to members of the genus Aminiphilus (89% similarity) in the family Aminiphilaceae of the phylum Synergistetes, and represented a novel genus-level clade, previously known as EBM-39. Fermentative growth with various amino acids, propionate and some specific sugars was observed, with acetate and hydrogen as the main end products. Syntrophic growth occurred with propionate, butyrate and caprylate in the presence of an H2-utilizing methanogen, and growth on butyrate and caprylate was only observed under syntrophic conditions. Therefore, this strain may play roles as both secondary fermenter and biomass scavenger in the engineered anaerobic bioreactors/digestors and laboratory enrichments, where primary fermentation products and necromass are readily available. Based on chemotaxonomic, phylogenetic and genomic characteristics, strain L-13T (=CGMCC 1.17908) was identified and nominated as Aminirod propionatiphilus gen. nov., sp. nov.
KW - Bioreactor
KW - Digestor
KW - Methanogenic hydrocarbon degradation
KW - Secondary fermenter
KW - Synergistetes
KW - Syntrophic metabolism
UR - http://www.scopus.com/inward/record.url?scp=85114706426&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2021.105323
DO - 10.1016/j.ibiod.2021.105323
M3 - 文章
AN - SCOPUS:85114706426
SN - 0964-8305
VL - 165
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
M1 - 105323
ER -