@article{3473a1b3084d4433979b2966b5b4710f,
title = "Aeromonas hydrophila AH-3 AexT is an ADP-ribosylating toxin secreted through the type III secretion system",
abstract = "We cloned and sequenced an ADP-ribosylating toxin (AexT) from a mesophilic Aeromonas hydrophila strain AH-3 with a type III secretion system (T3SS). This toxin only showed homology, in genes and proteins, with the first half of A. salmonicida AexT. The A. hydrophila AexT showed ADP-ribosyltransferase activity, translocation through the T3SS system, and this A. hydrophila T3SS system is inducible under calcium-depleted conditions. The A. hydrophila aexT mutant showed a slight reduction in their virulence assayed by several methods when compared to the wild-type strain, while an A. hydrophila T3SS mutant is highly reduced in virulence on the same assays. The A. hydrophila AexT is the first described and the smallest T3SS effector toxin found in mesophilic Aeromonas with a functional T3SS.",
keywords = "AexT toxin, Mesophilic Aeromonas, Type III secretion, Virulence",
author = "Silvia Vilches and Markus Wilhelms and Yu, {Hong Bing} and Leung, {Ka Yin} and Tom{\'a}s, {Juan M.} and Susana Merino",
note = "Funding Information: This work was supported by Plan Nacional de I+D and FIS grants (Ministerio de Educaci{\'o}n, Ciencia y Deporte and Ministerio de Sanidad, Spain) and from Generalitat de Catalunya. SV, and MW are predoctoral fellowships from Generalitat de Catalunya, and Ministerio de Educaci{\'o}n, Ciencia y Deporte. We are grateful to the Biomedical Research Council of Singapore (BMRC), A*STAR, and the National University of Singapore for providing research grants to K.Y.L. in supporting of this study. We thank Prof. J. Frey (Switzerland) for kindly providing polyclonal antiserum against A. salmonicida AexT, and Dr. Allain Filloux (France) for providing P. aeruginosa strain PA103. We also thank Maite Polo for her technical assistance.",
year = "2008",
month = jan,
doi = "10.1016/j.micpath.2007.06.004",
language = "英语",
volume = "44",
pages = "1--12",
journal = "Microbial Pathogenesis",
issn = "0882-4010",
publisher = "Academic Press Inc.",
number = "1",
}
