TY - JOUR
T1 - A numerical study on the loading of cryoprotectant cocktails-on-a-chip, Part I
T2 - Interacting miscible viscous fluids
AU - Scherr, Thomas
AU - Pursley, Shelby
AU - Todd Monroe, W.
AU - Nandakumar, Krishnaswamy
N1 - Funding Information:
We acknowledge support from the National Science Foundation ARI-R2 program Grant CMMI-0963482 . Thomas Scherr is supported by the National Science Foundation Computational Fluid Dynamics IGERT at Louisiana State University, as well as a Clayton Engineering Excellence Award for Outstanding Graduate Student at Louisiana State University. He would also like to acknowledge research funding from a Coates Research Grant at Louisiana State University. Shelby Pursley is supported by the Chancellors Future Leaders in Research program at Louisiana State University and a Clayton Engineering Excellence Award for Outstanding Undergraduate Student at Louisiana State University.
PY - 2014/11
Y1 - 2014/11
N2 - The laminar flow in microfluidic devices has shown promise as an effective delivery mechanism for cryoprotective agents to biological cells. For this method to gain more prevalence, its potential for use with more realistic and more complicated mixtures of cryoprotectants requires further exploration. In this work, we investigate the transport phenomena associated with a cryoprotectant cocktail consisting of 1.5 M 1,2-propanediol, 1.5 M dimethyl sulfoxide, and pure water. The viscous and miscible liquids are driven through a 25-cm long microchannel by a pressure gradient with inlet flow rate as the operating parameter. Our model resolves the spatially varying viscosity field, velocity field, and the concentrations of the three chemical species. With equal flow rates at each inlet, viscous sheets are formed and span the vertical direction of the channel. Depending on which cryoprotectant is introduced at the upstream inlets, the viscous sheets can move towards the center of the channel and be surrounding by less the less viscous mixture. This causes a unique velocity profile with three maxima in the transverse direction. As the inlet flow rates are decreased, the miscible liquids are afforded more time for molecular diffusion. Further decreasing the flow rate leads to a well-mixed viscous mixture of the three chemical species. The features of this cryoprotectant loading configuration are unique and, with additional optimization, could lead to improved cell survival rates during cryopreservation.
AB - The laminar flow in microfluidic devices has shown promise as an effective delivery mechanism for cryoprotective agents to biological cells. For this method to gain more prevalence, its potential for use with more realistic and more complicated mixtures of cryoprotectants requires further exploration. In this work, we investigate the transport phenomena associated with a cryoprotectant cocktail consisting of 1.5 M 1,2-propanediol, 1.5 M dimethyl sulfoxide, and pure water. The viscous and miscible liquids are driven through a 25-cm long microchannel by a pressure gradient with inlet flow rate as the operating parameter. Our model resolves the spatially varying viscosity field, velocity field, and the concentrations of the three chemical species. With equal flow rates at each inlet, viscous sheets are formed and span the vertical direction of the channel. Depending on which cryoprotectant is introduced at the upstream inlets, the viscous sheets can move towards the center of the channel and be surrounding by less the less viscous mixture. This causes a unique velocity profile with three maxima in the transverse direction. As the inlet flow rates are decreased, the miscible liquids are afforded more time for molecular diffusion. Further decreasing the flow rate leads to a well-mixed viscous mixture of the three chemical species. The features of this cryoprotectant loading configuration are unique and, with additional optimization, could lead to improved cell survival rates during cryopreservation.
KW - Cryopreservation
KW - Microfluidics
KW - Miscible fluids
KW - Numerical modeling
KW - Viscous flows
UR - http://www.scopus.com/inward/record.url?scp=84907599762&partnerID=8YFLogxK
U2 - 10.1016/j.ijheatmasstransfer.2014.07.026
DO - 10.1016/j.ijheatmasstransfer.2014.07.026
M3 - 文章
AN - SCOPUS:84907599762
SN - 0017-9310
VL - 78
SP - 1284
EP - 1291
JO - International Journal of Heat and Mass Transfer
JF - International Journal of Heat and Mass Transfer
ER -